Modifying Enzymes
New England Biolabs, Inc. Endonuclease IV – 1000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
New England Biolabs, Inc. T4 Polynucleotide Kinase (3' phosphatase minus) – 200 units
T4 Polynucleotide Kinase catalyzes the transfer and exchange of P from the gamma position of ATP to the 5 -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3'-monophosphates. This modified version exhibits full kinase activity with no 3' phosphatase activity.
New England Biolabs, Inc. AMV Reverse Transcriptase – 200 units
Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
New England Biolabs, Inc. T5 Exonuclease – 5000 units
T5 Exonuclease degrades DNA in the 5 to 3 direction. T5 Exonuclease is able to initiate nucleotide removal from the 5 termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
New England Biolabs, Inc. Human Alkyladenine Glycosylase (hAAG) – 500 units
hAAG excises alkylated and oxidative DNA damaged sites and catalyzes the hydrolysis of the N-glycosidic bond to release the damaged base.
New England Biolabs, Inc. CviKI-1 – 250 units
A restriction endonuclease that recognizes the sequence RG^_CY. CviKI-1 is a restriction endonuclease with 4 expected recognition sites as well as up to eleven relaxed non-cognate sites (star sites).
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
Macherey-Nagel Liquid RNase A, 100 mg/mL Concentration, Ready-to-Use Liquid, 2.5 mL
MACHEREY-NAGEL Liquid RNase A is a ready-to-use enzyme solution designed for RNA digestion in molecular biology. This highly concentrated RNase A facilitates efficient sample preparation for cell lysis and nucleic acid digestion, eliminating the need for prior dissolution. Its compatibility with NucleoSpin™ and NucleoBond™ kits ensures streamlined workflows. With a concentration of 100 mg/mL and a shelf life of 24 months, it stands out for its quality and effectiveness.
Pfaltz & Bauer Taka-Diastase | 9001-19-8 | MFCD00081319 | 100g
Taka-Diastase | 9001-19-8MFCD00081319100g
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 10000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Lambda Protein Phosphatase – 20000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.